The inhibition of growth indicates a negative result. The following is the composition of the cetrimide agar: Woods or UV light (360 nm) or short-wavelength (254-nm) UV light. (-) = non-lactose fermenters, colorless (or very faint pink) growth. Pseudomonas aeruginosa produces a number of water soluble iron chelators, including the yellow-green or yellow-brown fluorescent pyoverdin. 41 A leg culture from a nursing home patient grew gram negative rods on from TRAUMA 123 at St. Scholastica's College Manila USP <62> says to inoculate the selective media with an inoculum less than or equal to 100 CFU. -`gx`/y_R@@]3j\`4P When transfer organism from vial to a Petri dish should the vial be flam? Bacteria trapped in crystal inclusions found 'alive' after 50,000 years - what were they eating all that time? XLD agar is composed of yeast extract, sodium chloride, xylose, lactose, sucrose, l-lysine hydrochloride, sodium thiosulfate, iron (III) ammonium citrate, phenol red, sodium deoxycholate, agar, and distilled or deionized water. The difference between the phonemes /p/ and /b/ in Japanese, Difficulties with estimation of epsilon-delta limit proof. What kind of microorganisms can XLD be used for? Why or why not? For instance, if Tryptic Soy Agar (TSA) and MacConkey Agar are tested in parallel from an Escherichia coli suspension containing 100 CFU per inoculum, the E. coli will usually recover more colonies the nutrient-rich TSA than on MacConkey. The boiled medium is then distributed into tubes and sterilized in an autoclave at 15 lbs pressure (121C) for 15 minutes. Xylose Lysine Deoxycholate agar (XLD agar) is a selective growth medium used in the isolation of Salmonella and Shigella species from clinical samples and from food. EZ-Accu Shot, EZ-Accu Shot Select, EZ-CFU and EZ-CFU One Step are designed to make the test hassle-free. Pseudomonas gives negative Voges Proskauer, indole and methyl red tests, but a positive catalase test. 0 Learn how your comment data is processed. International Centre for Diarrhoeal Disease Research, Bangladesh . What is the main role of glycerol in cetrimide agar. It only takes a minute to sign up. Made with by Sagar Aryal. There is a reason why it has been used for the last 65 years. Do you have any clue about what could be the responsible for the different results observed? I have question regarding Cetrimide agar. This would serve as a means of performing quality control on your entire pre-enrichment, enrichment and detection system, whether the detection system be molecular or culture based. Differential in nature since it allows the growth of P.aeruginosa specifically and not any other Pseudomonas species, Maybe there was error in your serial dilutions. By clicking Post Your Answer, you agree to our terms of service, privacy policy and cookie policy. https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies.jpg. Any answers or links to relative resources are greatly appreciated! Elsevier. Is this indicating the positive results of P.aeroginosa? Cetrimide agar is used to determine the ability of an organism to grow in the presence of cetrimide, a toxic substance that inhibits the growth of many bacteria by causing the release of nitrogen and phosphorous, which slows or kills the organisms because organisms other than P. aeruginosa are unable to withstand this germicidal activity, while I have a question regarding the different TSA brands quality. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. <<1987180F52182F44A720D2DD0249EA65>]>> She also earned a medical technology degree from Fairview General Hospital. Is anyone enriching the organisms first? organisms: Ps. Cetrimide Agar can be bought commercially in the form of dehydrated powder. nareshchand02@gmail.com Pink rods Why glycerol. Thanks for contributing an answer to Biology Stack Exchange! Anupama Sapkota has a bachelors degree (B.Sc.) She graduated from Case Western Reserve University with a degree in Biology. Legal. hbbd``b`Z$[AN vH,@M 2 @OH0)#L,Fn0 + Learn more about Stack Overflow the company, and our products. xref Could you put the organism straight on the broth soaked sterile pad or would it be best to run it through a filter and transfer the filter onto the broth pad? Occasionally some enterics will exhibit a slight yellowing of the medium; however, this coloration is easily. 0000001631 00000 n Macconkey agar plates protocols. Do you have a bioreactor? Whenever i spread less 100 CFU on the surface of selective media (like MSA , MCA, XLDA, there were no recovery observed in the plate , but same inoculum show growth when spread on non-selective agar media ( like SCDA). AV9\~lc+pk_C1_C\^@~-;DBvg9wb@_'@RGl[Wf|5F$ Some species of Streptococci do not produce hemolysins. Agar is the solidifying agent. document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); About Us - Contact Us - Privacy Policy & Disclaimer, Benedicts Test- Principle, Composition,, Widal Test- Introduction, Principle, Procedure,, Different Size, Shape and Arrangement of Bacterial Cells, Gram Staining: Principle, Procedure, Interpretation,, Nutrient Agar: Composition, Preparation and Uses, MacConkey Agar- Composition, Principle, Uses,, Catalase Test- Principle, Uses, Procedure, Result, Cetrimide Test Principle, Procedure, Uses and Interpretation, List of culture media used in microbiology with their uses, Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar- Composition, Principle, Uses, Preparation and Colony Morphology, Xylose Lysine Deoxycholate (XLD) Agar- Principle, Uses, Composition, Preparation and Colony Characteristics, It is primarily used for the selective isolation and presumptive identification of, It is also used for determining the ability of an organism to produce fluorescein and pyocyanin (Antibiotica). Purpose: Selective and differential medium; identification of Enterobacteriaceae. From the E. colis viewpoint, growing on TSA is like eating a well-balanced diet containing plenty of fruits and vegetables, whereas growing on MacConkey is like eating nothing but potato chips. (+) = Lactose fermentation, dark purple colonies with dark center. Another feature associated with Pseudomonas is the secretion of pyoverdin (fluorescein, a siderophore . 0000004899 00000 n ,4"65aa_5qh-vaa/7bXqgQGFF}:jjc*zc}1^99WMlxyI?Jk]?/~])MS'%'8%N\hMT8OMA)kRSwgXMOLJa8cfDfzYg%fZg=?$N#9rs]ms0oc~z\RMHRZu~8Jt_y%MyuyufyY \bK"8NsvTxRzM^m.S=eOQt*i4w`]pmZjXYB{+u?EO.Z/E?Zt`%g0EKp^8 ]x;SDNP9yU\W^/Dg\KCU. Give the results for the inoculated bacteria. Basic Protocol 1: Growth of E. coli from frozen stocks Basic Protocol 2: Growth of E. coli in liquid media Basic Protocol 3: Enumeration of E. coli on solid media Basic Protocol 4: Storage of E. coli frozen stocks in glycerol Basic Protocol 5: Storage of E. coli in agar stabs Basic Protocol 6: Growth curve of E. coli liquid culture Open Research Directions: Streak agar in a straight line and incubate for 24 48 hours. Can ps.aeruginosa viable for 12days (288hrs)of extended incubation on cetrimide agar. 0000023064 00000 n hYn8>1(fi(] h*}g(;I^ RXVF$ GK`7FID4q4Qa=g-dH!RqX229989#[X#U s1rv uiVt.%Dx'%}GY5.#p'HaT Result Interpretation on MacConkey Agar Lactose non-fermenting strains, such as Shigella and Salmonella are colourless and transparent and typically do not alter appearance of the medium. What is the reflection of the story of princess urduja? FIG. When in contact with bacteria, causes the release of nitrogen and phosphorous from the bacterial cellother than Pseudomonas aeruginosa. We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. Cetrimide agar positive (growth; yellow-green to blue pigment). 1. Why are Shigella colonies red in XLD agar? ]|O>@O[< 2Cp@ > endobj 43 0 obj<>/Encoding<>>>>> endobj 44 0 obj<>/ProcSet[/PDF/Text]>>/Type/Page>> endobj 45 0 obj[46 0 R] endobj 46 0 obj<>/AP<>>> endobj 47 0 obj<>/Type/XObject/BBox[0.0 0.0 352.407 32.5299]/FormType 1>>stream % Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. 0000029158 00000 n Why is XLD agar used for the isolation of Salmonella? Lack of growth on cetrimide agar doesnot rule out an identification of Pseudomonas aeruginosa. Laurie Kundrat, MT (ASCP), is a former Microbiologics employee and regular contributing author to the Microbiologics Blog. You can define the comparability criterion yourself. . PEA agar plates with 5% sheep blood: (a) an uninoculated PEA agar plate with 5% sheep blood, (b) a PEA agar plate with 5% sheep blood inoculated with Escherichia coli, a gram-negative bacteria, incubated under 5% CO 2 for 48 hr at 35 oC 2oC (growth inhibited), and (c) a PEA agar plate with 5% sheep blood inocul ated withStaphylococcus EFL[@z;tNCraY@&.|a9 HG; \65#iuaHUTFZ. Slight differences between the media formulations and the quality of the ingredients from different manufacturers can influence the recovery of the strain. Glycerol acts as the carbon source. Mechanism/reactions: Selects for Gram Negative bacteria, and differentiates those enterics which ferment lactose (coliforms) from those which do not ferment lactose (non-coliforms). Back to Basics: Best Practices for Growth in Liquid Media, De-complicating Incoming Inspection of Ready-to-Use Cultures, How to Perform Serial Dilutions in Microbiology, 0392A Aspergillus brasiliensis derived from ATCC 16404, Our Top Posts from 2017 Microbiologics Blog, 8 Best Practices for Growth Promotion Testing Microbiologics Blog, Growth Promotion Test Quiz Microbiologics Blog, Remember fungus prefers cooler temperatures. Would this decrease possible contamination?Would this damage the organism that are currently in the vial causing > 100cfu ( using TSA agar). She is particularly interested in studies regarding antibiotic resistance with a focus on drug discovery. Stack Exchange network consists of 181 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers. . 0000002129 00000 n The media further contains gelatin peptone that provides the necessary nutrients for, Sodium chloride maintains the osmotic equilibrium of the medium and prevents the change of pH of the medium during growth. She has a passion for working with customers and helping them use Microbiologics products successfully. We are doing soil testing for the presence of P.spp . For what it's worth, you might find minimal salts media and solid state fermentation interesting. agar with Lemco base (CTA 1) and cetrimide agar with a slightly modified King's base (CTA2) in the isolation of Ps. Staphylococcus aureus is a BSL2 organism. If growth is observed on a Cetrimide Agar plate, . Any advise? Cetrimide agar is a selective plate medium used occasionally to isolate Pseudomonas species from a mixed bacterial flora. What did the Nazis begin using gas chambers instead of mobile killing units and shooting squads after a while? For instance, you may need to incubate pour plates an extra 24 hours before you can see tiny Staphylococcus aureus colonies. Additional testing such as biochemical tests and serological procedures should be performed to confirm the findings and to confirm a diagnosis of, Microbial Culture Media- Definition, Types, Examples, Uses, Microbiology of Extreme Environments (Types and Examples), Carbapenem-Resistant Pseudomonas aeruginosa (CRPA), Bacteria- Definition, Structure, Shapes, Sizes, Classification, Tille P.M (2014)Bailey and Scotts diagnostic microbiology, Thirteen edition, Mosby, Inc., an affiliate of Elsevier Inc., 3251 Riverport Lane, St. Louis, Missouri 63043, https://microbeonline.com/cetrimide-agar-composition-principle-preparation-uses/, https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/CetrimideSelAgar.htm, Biopesticides- Definition, 3 Types, and Advantages, OF Test- Oxidation/Oxidative-Fermentation/Fermentative Test, Novobiocin Susceptibility Test- Principle, Procedure, Results, Nitrate Reduction Test- Principle, Procedure, Types, Results, Uses, Nosocomial Infections (hospital-acquired infections). Mannitol salt agar plates protocols. Web. He has published more than 15 research articles and book chapters in international journals and well-renowned publishers. x][\qW[v]!UJ9N,LAEJqI"*?1A_0CXw4p?Zl+8!)0"|0Ga Zk:`H LiH5Nf$P>8fL3R`qc`(X*X(f7"r#{[alTPD) T%7L Furo` D27ZK Zl)0 Agar is the solidifying agent. ), Purpose: Selective and differential; identification of pathogenic Staphylococci, Media: Mannitol Salt Agar (MSA) contains mannitol, 7.5% sodium chloride, and phenol red. 2023 Microbe Notes. Cetrimide also enhances the production of Pseudomonas pigments such as pyocyanin and pyoverdine, which show a characteristic blue-green and yellow-green colour . For instance, if Tryptic Soy Agar (TSA) and MacConkey Agar are tested in parallel from an Escherichia coli suspension containing 100 CFU per inoculum, the E. coli will usually recover more colonies the nutrient-rich TSA than on MacConkey. Making statements based on opinion; back them up with references or personal experience. How to Market Your Business with Webinars. Cetrimide is a quaternary ammonium salt, which acts as a cationic detergent that is toxic to most bacterial cells. Naresh Be careful to not only enter +/-, but also a short description of the colony characteristics on each media. 2% https://microbiologyinfo.com/cetrimide-test/, 1% https://www.slideshare.net/sayantanmondal96/identification-of-bacteria-35638850, 1% https://www.sciencedirect.com/topics/medicine-and-dentistry/achromobacter-xylosoxidans, 1% https://orbitbiotech.com/pseudomonas-aeruginosa-isolation-and-identification/, 1% https://microbiologynotes.com/cetrimide-test-principle-procedure-result-interpretation-and-limitation/, 1% https://assets.thermofisher.com/TFS-Assets/LSG/manuals/IFU1292.pdf, <1% https://www.who.int/water_sanitation_health/resourcesquality/wqmchap10.pdf, <1% https://www.techylib.com/en/view/mexicorubber/pathogenic_microbiology_college_of_computer_mathematical, <1% https://www.cram.com/flashcards/non-fermentative-gram-negative-rods-1568966, <1% https://biologicalindicators.mesalabs.com/wp-content/uploads/sites/31/2014/02/Unique-Cycles-Sterilizing-Liquid-Loads.pdf, Result and Interpretation of Cetrimide Agar Test, Biopesticides- Definition, 3 Types, and Advantages, OF Test- Oxidation/Oxidative-Fermentation/Fermentative Test, Novobiocin Susceptibility Test- Principle, Procedure, Results, Nitrate Reduction Test- Principle, Procedure, Types, Results, Uses, Nosocomial Infections (hospital-acquired infections). Microbial culture media is used in many industries to grow, enumerate, and identify microorganisms. WDCM 00034 . Improve this question. How can this new ban on drag possibly be considered constitutional? Cetrimide agar is a type of agar used for the selective isolation of the gram-negative bacterium, Pseudomonas aeruginosa. Cetrimide agar is used to determine the ability of an organism to grow in the presence of cetrimide, a toxic substance that inhibits the growth of many bacteria by causing the release of nitrogen and phosphorous, which slows or kills the organisms because organisms other thanP. aeruginosaare unable to withstand this germicidal activity, while P. aeruginosa is resistant to cetrimide. Colonies often grow more slowly on pour plates compared to spread plates. Kathy Generally, Growth Promotion Testing is conducted directly on the agar plates and/or in the broth bags (or tubes) prior to their use in the laboratory. It can also be prepared in the lab if the necessary constituents of the media are available. The Cetrimide plate is specifically for Pseudomonas aeruginosa organisms only as it is a selective in nature. A rather long list that I won't post here can be found at http://structuralbiology.uchc.edu/protocols/pdfs/nmr_sample_preparation.pdf. Hello, A background light can help you spot them. Sterilize by autoclaving at 15lbs pressure (121C) for 15 minutes. The test works on the principle of the ability of an organism to grow in the presence of cetrimide. 8198033938. Escherichia coli and Campylobacter jejuni are some of the common causes of bacterial gastroenteritis. "+" for growth/utilization; "-" for weak or no growth/utilization: E. coli inactive** is lactose-negative, non-motile- often misidentified as Shigella. You could add some glucose . As the name suggests, it contains cetrimide, which is the selective agent against alternate microbial flora. The tubes are then incubated aerobically at 35-37C for up to 7 days. Is the singer Avant and R Kelly brothers? Laurie is an active member of the Personal Care Products Council (PCPC) and serves as a member of the Microbiology Committee. If determining the concentration of an unknown microorganism sample, you may find it useful to reference our dilution guide video on our website here. where MacConkey Agar is used to screen for Escherichia coli. By using a standardized inoculum of 10-100 CFU, you can avoid the unpleasant surprise of finding 120 colonies on your agar plate the day after you inoculated it with the suspension prepared with a turbidimeter. Sterilize by autoclaving at 121C for 15 minutes. Good information to consider when doing GPT! Learn how your comment data is processed. A negative reaction is denoted by no growth. Is it possible to make anaerobic media too reducing? 1 October 2016. Web. To subscribe to this RSS feed, copy and paste this URL into your RSS reader. It is an oxidase-positive, gram-negative rod varying in size from 0.5 to 0.8 m by 1.5 to 3.0 m. [WH9[&>)eJOfMVev)XMi] ]&_ynGG!(*Gv 00i H = ` d.g-~FEwLx0;2p Cool to 45-50C. Why is MSA optional only during the unknowns? Purpose: Selective and differential medium; identification of Enterobacteriaceae. Beware of hot spots in your incubator. Cetrimide agar is primarily used for selective isolation and presumptive identification ofPseudomonas aeruginosa from clinical and nonclinical specimens. It is lactose-fermenting and beta-hemolytic on blood agar. Growth on this medium alone is not sufficient for identification of Pseudomonas aeruginosa to the species level, since other non-glucose-fermenting species may grow. The test tubes should be examined daily for 4 days and again at 7 days before discarding the result as a negative. .KwB&,gy$7c.#K/>/)ldicd#c@,B44a0F}FMX&j/-C3) fB}*Wf)76t. Gelatin peptone provides necessary nutrients for P. aeruginosa. Pseudomonas aeruginosa ATCC 9027 Yellow-green to blue colonies.Escherichia coli ATCC 8739 Partial to complete inhibition. MacConkey Agar contains lactose, which E. coli can ferment for energy, . It is also used to detect the ability of an organism to tolerate cetrimide and exhibit growth on cetrimide agar. The agar plates need to reach 30C to 35C quickly to give the bacteria enough time to grow. 6 Why are Shigella colonies red in XLD agar? Cetrimide is a quaternary ammonium salt, which acts as a cationic detergent that reduces surface tension in the point of contact and has precipitant, complexing and denaturing effects on bacterial membrane proteins. Q.C. As for your question on streaking, if you mean streaking with one or more colonies, you shouldnt do it if you follow USP standards. Use the environmental conditions required by the species. Cetrimide agar in dehydrated from his available from Gibco Ltd and other manufacturers of culture media. Preparation and Method of Use of Tryptic Soy Agar Suspend 45 grams in 1000 ml distilled water. Hence, it is used as a selective medium for the isolation ofPseudomonas aeruginosafrom various clinical specimens. Some species of Citrobacter and Enterobacter will also react this way to EMB. endstream endobj 48 0 obj<> endobj 49 0 obj<> endobj 50 0 obj<> endobj 51 0 obj<> endobj 52 0 obj<> endobj 53 0 obj<> endobj 54 0 obj<> endobj 55 0 obj<> endobj 56 0 obj<> endobj 57 0 obj<>stream Mannitol is fermented by Staphylococcus aureus and will cause a pH change in the medium (acidic) that is detected by observing phenol red changing to yellow. . Colonies exhibiting fluorescence at 250nm and a blue-green pigmentation are considered as presumptive positive. Laurie Kundrat, MT (ASCP) has over 25 years of experience as a Microbiologist and a Clinical Technologist. A member of the Enterobacteriaceae, it grows well on blood or MacConkey agar and in nutrient broths, such as brain-heart infusion. Weak fermenters will have pink mucoid growth. She graduated from Case Western Reserve University with a degree in biology. To learn more, see our tips on writing great answers. E. coli on XLD Agar Partial to complete inhibition; yellow to yellow-red colonies. Made with by Sagar Aryal. Limitation of Cetrimide Test. 0000004635 00000 n 0000001473 00000 n 0000030106 00000 n The colour of E coli is green metallic sheen colour on EMB agar. Cetrimide agar test is a biochemical test performed to identify or differentiate Pseudomonas aeruginosa from other microorganisms. Used for the isolation of Pseudomonas aeruginosa from pharmacological preparations. Microbial Culture Media- Definition, Types, Examples, Uses, Bacteria- Definition, Structure, Shapes, Sizes, Classification, Carbapenem-Resistant Pseudomonas aeruginosa (CRPA), Microbiology of Extreme Environments (Types and Examples). Mechanism/reactions: Salt concentration will inhibit most other organisms so the media is selective for staphylococci. Glycerol is supplemented as a source of carbon. Routing number of commercial bank of Ethiopia? When cetrimide is in contact with bacteria, nitrogen and phosphorus are released from the bacterial cell. What does E coli look like . Cetrimide Agar Growth promoting P. aeruginosa Inhibitory E. coli simple method is that set id SIMCUT,90% you can identify E.coli. I havent been able to find anything conclusive. I have question regarding Cetrimide agar. 4 What bacteria can grow on Cetrimide Agar? Xylose Lysine Deoxycholate (XLD) Agar is a selective medium for the isolation of Salmonella and Shigella spp from clinical specimens and food samples. Composition of centrimide agar 0000032632 00000 n v5x3Qx2Nm K[qdYqU[BiI2c Primarily the usage of XLD agar is for the detection of Salmonella species in food analysis. 0000024780 00000 n Unit 22: Physiological Tests for Characterization and Identification of Bacteria, Bio 221Lab: Introduction to Microbiology (Burke), { "22.01:_Learning_Objectives" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "22.02:_Selective_and_Differential_Media_-_MacConkey_EMB_MSA" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "22.03:_Chromogenic_Media" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "22.04:_Blood_Agar_Plates_(BAP)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "22.05:_Fermentation_and_Utilization_Media-Durham_Sugar_Tubes_MRVP_Oxidase_Catalase_Citrate" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "22.06:_Hydrolytic_and_Miscellaneous_Media_-_Starch_Skim_Milk_Gelatin_Indole_Urea_Kliglers_TSI" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "01:_Safety" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "02:_The_Metric_System_Measurement_and_Lab_Equipment_Review" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "03:_Microscopy" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "04:_Environmental_Sampling" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "05:_Survey_of_Eukaryotic_Microorganisms-_The_Protists_Algae" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "06:_Parasitic_Helminths" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "07:_Fungi" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "08:_Pure_Cultures-_Aseptic_Transfer_Techniques_and_Streak_Plates_for_Isolation" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "09:_Bacterial_Growth_Patterns-_Building_your_Stock_Cultures_and_Observing_Culture_Characteristics" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "10:_Bacterial_Growth_Patterns-_Direct_Count_The_Standard_Plate_Count_and_Indirect_Turbidimetric_Methods" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "11:_Environmental_Effects_on_Growth-_Temperature" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "12:_Environmental_Effects_on_Growth-_pH" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "13:_Environmental_Effects_on_Growth-_Osmotic_Pressure" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "14:_Oxygen_Requirements-_FTM_and_the_Anaerobe_Jar" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "15:_Environmental_Effects_on_Growth-_Antimicrobial_Sensitivity_Testing" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "16:_Transformation(1)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "17:_Smear_Prep_and_Simple_Stains" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "18:_Negative_Stain" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "19:_Gram_Stain" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "20:_Endospore_Stain" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "21:_Acid-Fast_Stain-_Kinyoun_Method" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "22:_Physiological_Tests_for_Characterization_and_Identification_of_Bacteria" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "23:_Unknown_1_-_What_is_yellow_wrinkled_round" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "24:_Unknown_2-__Mixed_Culture" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "25:_Bacterial_Examination_of_Food-_Standard_Plate_Counts" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "26:_Bacterial_Examination_of_Water-_Multiple_Tube_Test_Standard_Plate_Count_and_Membrane_Filter_Technique" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "27:_Immunology-_ELISA-Simulation_StaphTEX-Agglutination_Reaction" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "28:_Microbescopes_and_Observation_of_Natural_Samples" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "zz:_Back_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, 22.2: Selective and Differential Media - MacConkey, EMB, MSA, [ "article:topic", "showtoc:no", "license:ccby", "program:ztccoc", "authorname:ckberke" ], https://bio.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fbio.libretexts.org%2FCourses%2FCollege_of_the_Canyons%2FBio_221Lab%253A_Introduction_to_Microbiology_(Burke)%2F22%253A_Physiological_Tests_for_Characterization_and_Identification_of_Bacteria%2F22.02%253A_Selective_and_Differential_Media_-_MacConkey_EMB_MSA, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), http://www.asmscience.org/content/education/protocol/protocol.2855, http://www.asmscience.org/content/education/protocol/protocol.2869, http://www.asmscience.org/content/education/protocol/protocol.3034, College of the Canyons - Zero Textbook Cost Program, status page at https://status.libretexts.org.